9/29/2017 0 Comments Ssh Keygen Pem To Ppk FileCommand- line Options : : Win. SCPParameters. Parameters for winscp. Parameters for winscp. Session. The first syntax opens the site. To open site, stored in folder, use path syntax “folder/site”.
You can also open workspace or all sites in site folder. Advertisements. The second creates the session specified by session URL and optionally by initial remote path. If the remote path is not ended by slash (/), it is treated as path to file (or even directory) that should be downloaded. Parameter /sessionname specifies a custom name of the session to be used instead of the automatically generated name in a format username@hostname or to override the name of the saved site. If there’s already idle Win. Frequently Asked Questions for Virtual Machines How to enable the SSH server? The SSH server is disabled by default in Bitnami virtual machines. Alternatively if you want to grab the private and public keys from a PuTTY formated key file you can use puttygen on *nix systems. For most apt-based systems puttygen. SCP instance running, the session(s) opens in the existing instance. To force session open in new instance of Win. SCP, use /newinstance parameter. Parameter /privatekey specifies local path to SSH private key file. Parameter /hostkey specifies fingerprint of expected SSH host key (or several alternative fingerprints separated by semicolon). It makes Win. SCP automatically accept host key with the fingerprint. Parameter /clientcert specifies local path to FTPS or Web. DAVS TLS/SSL client certificate. When the FTPS or Web. DAVS server TLS/SSL certificate is not trusted (typically a self- signed certificate), use parameter /certificate to specify a fingerprint of the untrusted certificate. It makes Win. SCP trust the certificate. Several alternative fingerprints can be separated by semicolon. Parameter /passive enables passive (=on) or active (=off) transfer mode (FTP protocol only). Parameters /implicit, and /explicit enable respective method of invoking FTPS. Parameter /timeout specifies server response timeout. Parameter /rawsettings allows configuring any site settings using raw format as in an INI file. E. g. to enable SSH compression and agent forwarding use /rawsettings Compression=1 Agent. Fwd=1. The parameter must come after session URL (if any). When using scripting, use open command (and its switches) instead. My site". winscp. Logging. With /log parameter you may turn on session logging to file specified by local path. Use parameter /loglevel to change logging level. The value can be in range 0. Normal, Debug 1 and Debug 2 logging levels respectively). Append additional * to enable password logging (e. Use parameter /logsize to configure log file size limit and log file rotation. Specify maximum size in bytes, optionally with K, M or G units. Optionally you can limit number of archived log files using count* prefix. For example /logsize=5*1. M will limit log file size to 1. MB and will allow up to 5 archived logs. C: \winscp. log" /loglevel=0. With /xmllog parameter you may turn on XML logging to file specified by local path. In either path you can use the same patterns as in the logging preferences. Use parameter /xmlgroups along with /xmllog, to group all XML log elements belonging to the same command under parent group element. Console/scripting mode. Parameter /console executes Win. SCP in console (scripting) mode. Note that when using winscp. To run batch script either pass script file using /script parameter or specify the commands directly on command line using /command. In the latter case each following parameter is treated as single command. See syntax section and examples below for details how to deal with spaces and double- quotes. If both /script and /command parameters are used, commands from script file are executed first. When the last command is not exit, regular non- batch mode follows. Use parameter /parameter to specify list of arguments to be passed to script. It is recommended to escape the arguments with // switch. With winscp. exe, if /console parameter is not used along with /script or /command, the script/command is executed without visual feedback (window). Use parameter /nointeractiveinput, when feeding commands to winscp. Also prevents error message popping up when fatal error occurs while starting Win. SCP. When combined with /xmllog the fatal error is recorded in the XML log. C: \Users\martin\Documents\myscript. You can have Win. SCP generate a scripting command- line for you. The following parameters can be used to create a shortcut that initiates operation in GUI mode. They are not intended for automation, for that see scripting. Use /edit to open a remote file in Win. SCP internal editor. With /synchronize or /keepuptodate parameter Win. SCP performs Synchronize or Keep remote directory up to date commands respectively on the specified session and directories. A dialog to set options is displayed first. With /upload parameter Win. SCP uploads specified files to initial remote directory of session. A dialog to set options is displayed first. Use /defaults parameter along with /upload, /synchronize or /keepuptodate to skip the settings dialog and start the operation straight away with default settings. Use the /refresh parameter to reload remote panel of all running instances of Win. SCP. If a session is specified on command- line, only instances that have that session as active are refreshed. If a path is specified after the /refresh, only that directory is refreshed. It is recommended to escape the arguments with // switch. C: \Users\martin\Documents\My. Site" /home/martin/public_html. Configuration. With /ini parameter you may specify local path to configuration INI file. It effectively disables using registry as configuration storage. If the file does not exist, default configuration will be used and the file will be created. C: \Users\martin\Documents\myconfig. Use nul instead of path to force Win. SCP start with its default configuration and not save the configuration on exit. With /rawconfig parameter you can set any configuration settings using raw format as in an INI file. E. g. to configure an external IP address use /rawconfig Interface\External. Ip. Address=1. 98. The parameter must come after a session URL (if any). The configuration set this way is preserved. Mass- modification of sites. Use /batchsettings to mass- modify stored sites. The first argument is a mask to select sites to modify. Use a syntax of basic file masks. You can also use path mask to select sites based on their folders. The other arguments define new values for site settings. Use the same syntax as for /rawsettings. For example to configure a proxy for all sites in a “clients” folder, use. Proxy. Method=3 Proxy. Host=proxy. Private key conversion and modification. Use the /keygen switch to convert private keys from other formats to a Pu. TTY . ppk format or to change their passphrase or comment. A parameter after the /keygen switch specifies a path to an input private key file. The input key can be in Open. SSH or ssh. com format (when converting the key to the Pu. TTY format) or in the Pu. TTY format (when changing a key passphrase or comment). When converting the key from other format, you need to specify an output key path using the /output switch. When modifying a Pu. TTY key, the existing file is overwritten, if /output is not specified. Use /changepassphrase switch to change the key passphrase. Use /comment switch to change the key comment. For example, to convert key mykey. Open. SSH format to mykey. Pu. TTY format and set its comment. Converted from Open. SSH format". To change the passphrase of existing mykey. For a compatibility with *nix puttygen, the - o, - P and - C switches are understood as aliases to /output, /changepassphrase and /comment respectively. So, for features supported by Win. SCP, you can use the same arguments as for puttygen, just prefixed with /keygen. Converted from Open. SSH format"Auxiliary. When run with /update parameter, Win. SCP only checks for its updates. Parameter /help shows usage (overview similar to this). Command- line parameters that include space(s) must be surrounded by double- quotes. C: \Users\martin\Documents\myconfig. To use the double- quote as a literal, use two double- quotes sequentially. For example, the /command expects that each script command is surrounded by double quotes, so that it is passed as a single command- line argument. In addition, any script command argument that includes spaces is expected to be surrounded by double- quotes within the command (see doubling double- quotes). C: \Users\martin\Documents\my file. Script command 1 - > < - Script command 2 - >. To debug the quoting, enable session logging on level Debug 1 (/loglevel=1). The log will show how Win. SCP understands your command- line. An argument that begins with a slash is considered a switch.
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Anticancer Activity of Novel Plant Extracts from Trailliaedoxa gracilis (W. W. Smith & Forrest) in Human Carcinoid KRJ- I Cells. BERNHARD SVEJDA1. VICTOR AGUIRIANO- MOSER1. SONJA STURM2. HARALD HÖGER3. ELISABETH INGOLIC4. VERONIKA SIEGL1. HERMANN STUPPNER2 and. ROSWITHA PFRAGNER1. Department of Pathophysiology and Immunology, Center of Molecular Medicine, Medical University of Graz, Graz, Austria. Institute of Pharmacy, Center of Molecular Biosciences, Leopold Franzens University of Innsbruck, Innsbruck, Austria. Core Unit of Biomedical Research, Division of Laboratory Animal Science and Genetics, Medical University of Vienna, Himberg. Austria. 4. Research Institute for Electron Microscopy and Fine Structure Research, University of Technology Graz, Graz, Austria. Correspondence to: Professor Roswitha Pfragner, Department of Pathophysiology and Immunology, Medical University of Graz, Heinrichstrasse 3. A- 8. 01. 0 Graz, Austria. Tel: +4. 3 3. 16. Fax: +4. 3 3. 16. Abstract. Background: Small intestinal (SI) neuroendocrine tumors (NETs) are rare neoplasms derived from neuroendocrine cells presenting. Nevertheless, many are asymptomatic and misdiagnosed. As response rates to chemotherapy are low, surgery remains the only effective treatment. Because many tumors have metastasized. Consequently, a substantial need for new therapeutic options. Materials and Methods: The effects of novel plant extracts from Trailliaedoxa gracilis (W. W. Smith & Forrest). ![]() The cytotoxic activity of 23 crude methanol. activity of selected Bangladeshi medicinal plant extracts. nor anticancer activity studies of D. In vitro anticancer activity of extracts. Mentha is a plant with worldwide distribution and. recorded on ELSIA reader at 540 nm. Suitable. IN-VITRO CYTOTOXICITY ACTIVITY OF SOLANUM NIGRUM EXTRACT AGAINST HELA CELL LINE AND VERO CELL LINE. Preparation of plant extracts. INVITRO ANTICANCER ACTIVITY OF RUBIA CORDIFOLIA AGAINST HELA AND. usage of plant extracts. to the filters available for the ELISA reader, used. Density at 570 nm using a plate reader. Both extracts showed less anticancer activity against. compounds from the plant that showed anticancer activity. SI- NET cell line KRJ- I and in KRJ- I transplanted mice. Proliferation and viability were analyzed. WST- 1 cell proliferation assay. Apoptosis was determined by DAPI staining and electron microscopy. ![]() Results: Extracts of Trailliaedoxa gracilis showed. KRJ- I cells. Normal fibroblasts were not impaired. Tumor growth inhibition was also observed in heterotransplanted SCID (severe combined immunodeficiency) mice. Conclusion. The in vitro and in vivo outcomes suggest a potential clinical effect of Trailliaedoxa gracilis in SI- NETs. Small intestinal (SI) neuroendocrine tumors (NETs) are rare neoplasms originating from neuroendocrine cells that are part. These tumors are characterized by malignant potential and slow growth with an annual incidence of 4- 6 per 1. Due to increasing clinical awareness and more wide- spread use of diagnostics, e. CT, MRI and the measurement of biochemical markers, the incidence of SI- NETs has increased annually. Diagnosis is currently based on the typical. Specific for the ileal carcinoid tumors are increased serum levels of serotonin, neuropeptide K and substance P (3, 4). These bioactive substances are responsible for the characteristic clinical symptoms of the carcinoid syndrome characterized. However, many tumors are so- called non- functioning causing no symptoms by the. Therefore a delay of diagnosis (5- 7 years) is typical, increasing the probability of liver metastasis (3) and consequently, more than 8. Surgery remains the only curative. Therefore, clinical management focuses mainly on the treatment of clinical symptoms using somatostatin analogues and interferon- α. While biotherapy with somatostatin and interferon- α appears to have unquestioned tumoristatic properties, these agents do. Recently, single- agent and multi- agent chemotherapy regimens have been evaluated with response rates from 0- 2. Due to the unchanged prognosis for SI- NETs over the past 3. For centuries, plants have been used in traditional medicines for the treatment of different diseases. In recent years, oriental. Several chemotherapeutic drugs derived from plants, such as Vinblastine, Taxol, Camptothecin and Podophyllotoxin are used. Using modern analytical and chemical techniques, novel natural compounds from herbs can be isolated by fractionation and. It has been estimated that only 5- 1. To study new therapeutic approaches, cell lines are used to investigate novel compounds and their effects on the tumor. However, few cell lines from human SI- NETs have been maintained so far, namely GOT1 (1. CNDT2 (1. 1), and therefore, we established four continuous tumor cell lines (KRJ- I, P- STS, L- STS, H- STS) (1. KRJ- I and P- STS being derived from primary ileal carcinoids. All established cell lines were investigated morphologically and immunocytochemically and established to derive from an SI- NET. After cell lines were used as cancer models to investigate antiproliferative activities of novel compounds, findings were. In recent studies, we have demonstrated a dose- dependent antitumor effect of bioactive agents derived from Cautleya gracilis (Smith) Dandy and extracts of Stemona tuberosa Lour with induction of apoptosis on medullary thyroid carcinoma cell lines established in our labaratory (1. Belonging to the family of Rubiaceae, like other Chinese herbs, Trailliaedoxa gracilis (W. W. Smith & Forrest) was found and described by the Austrian botanist Handel- Mazzetti (2. In a previous screening, an antiproliferative effect of T. W. W. Smith & Forrest) was noted in the medullary thyroid carcinoma cell lines MTC- SK (2. SINJ (2. 2). However, the bioactivity of T. Thus, the aim of the present study was to investigate for the first time antiproliferative activity in vitro using the SI- NET cell line KRJ- I and in vivo in xenotransplanted mice. Materials and Methods. Plant extracts and chemicals. The plant material (whole plant) was provided by E. Stöger (Oberndorf, Austria). A voucher specimen was deposited at the. Institute of Pharmacy/Pharmacognosy at the University of Innsbruck. The dried material was ground and macerated with dichloromethane. DCM), filtered and the solvent evaporated under reduced pressure (TG- 5). The extract was then re- dissolved in water/methanol. TG- 1), DCM (TG- 2) and ethylacetate (Et. OAc) (TG- 3) was performed. After evaporation of the solvents under reduced. DMSO, Sigma- Aldrich, Vienna, Austria) at a concentration of. C. The fractionations of TG- 5 and the subsequent subfractions were obtained by gel chromatography. Sephadex LH- 2. 0 column and DCM: acetone (8. Ursolic acid was crystallized from. TG- F2. 8 and the structure elucidation was carried out by 1. D and 2. D NMR and MS. The concentration of ursolic acid in TG- F2. H- NMR in DMSO- d. The fifteen obtained subfractions of T. KRJ- I cells and on fibroblasts. The concentrations of each fraction and subfraction. IC5. 0 values. Camptothecin (CPT) was purchased from Sigma- Aldrich (Sigma- Aldrich, Vienna, Austria), dissolved in DMSO and stored at a concentration. M at 4°C. Cell lines and cell culture. A continuous cell line from a human malignant carcinoid of the small intestine, KRJ- I (1. HF- SAR (2. 3) were established in our laboratory. The KRJ- I cells had been characterized histologically and biochemically to be derived. All the cell lines were Mycoplasma- free. The KRJ- I cells were cultured in Quantum 2. PAA Laboratories, Pasching, Austria). C, 5% CO2 and the HF- SAR were maintained in Eagle's MEM supplemented with 1. FBS at 3. 7°C, 5% CO2. For the in vitro experiments, the KRJ- I and HF- SAR cells were plated at an initial density of 2×1. Cell counting. KRJ- I cells were cultured in 2. Sarstedt, Wr. Neudorf, Austria) for 2. T. gracilis dissolved in DMSO. DMSO alone was used as negative control. The samples were suspended to disperse cell clusters into single. CASY®- 1 Cell Counter & Analyzer TTC (Schärfe System, Reutlingen, Germany). Each. sample was quantified four times and the mean of the sample and standard deviations (S. D.) were calculated by the cell counter. WST- 1 cell proliferation assay. Cell proliferation and viability were analyzed using the WST- 1 Cell Proliferation Reagent (4- [3- (4- iodophenyl)- 2- (4- nitrophenyl)- 2. H- 5- tetrazolio]- 1,3- benzene. Roche Diagnostics, Vienna, Austria) according to the manufacturer's instructions. The assay principle is based. The number of metabolically active cells correlates directly to the amount of formazan. The KRJ- I cells were cultured in 2. DMSO as negative control. After incubation, cell clusters were dispersed carefully into single cells. |
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